Recombinant adeno-associated virus expressing human antisense gene CyP2J2 and its preparation methods
US8273344B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 28, 2005 |
| Grant date | Sep 25, 2012 |
| Priority date | — |
| Expiry date | Mar 6, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14143
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A recombinant adeno-associated virus (rAAV) expressing antisense human CYP2J2 gene produced by a method of co-transfection into 293 cell by calcium phosphate precipitation, the rAAV having the following three plasmids: a) pXX2: a packing plasmid having a nucleotide sequence encoding AAV Rep and Cap protein, wherein the nucleotide sequence is operably linked upstream and downstream to a p5 promoter respectively to increase expression 15-fold; b) pXXUF1-anti2J2: an eukaryotic expression vector having a CMV promoter, a NotI site for insertion of target genes, the CYP2J2 gene oriented in the 3′ to 5′ direction, and inverted terminal repeats (ITRs); and c) pXX6: a helper plasmid deleted of the pathogenic gene sequences of adenovirus and having an E1A, an E2A, and a VA1 RNA gene of adenovirus. Also provided is a method of preparing the rAAV.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.