Method of amplifying a target nucleic acid by rolling circle amplification
US8278051B2 · kind B2 · utility
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22Claims
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Key dates
| Filing date | Jul 18, 2011 |
| Grant date | Oct 2, 2012 |
| Priority date | — |
| Expiry date | Jul 18, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.