Diagnosis of metal allergy through cytokine release by T-cells in vitro
US8278054B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 12, 2002 |
| Grant date | Oct 2, 2012 |
| Priority date | — |
| Expiry date | Sep 20, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/5406
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An in vitro method for diagnosing a contact allergy in a subject comprises: (i) providing (a) a contact allergen; (b) T-cells from said subject; and (c) a surface to which is immobilized a first specific binding agent capable of specifically binding to a cytokine released from a T-cell in response to said allergen; (ii) contacting said sample with said allergen under conditions which are suitable for (a) release of said cytokine by T cells that have been presensitized in vivo to said allergen; and (b) binding of said cytokine to said first specific binding agent; and (iii) detecting binding of said substance to said first specific binding agent. A kit which enables the method to be carried out is also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.