Molecule for assigning genotype to phenotype and components thereof as well as method for constructing assigning molecule and method for utilizing assigning molecule
US8278427B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 13, 2003 |
| Grant date | Oct 2, 2012 |
| Priority date | — |
| Expiry date | Nov 1, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1062
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An assigning molecule obtained by ligating a genotype molecule to a phenotype molecule by transpeptidation, wherein the genotype molecule is constructed by bonding (a) a spacer molecule comprising a donor region which can be bonded to a 3′-terminal end of nucleic acid, a PEG region bonded to the donor region and a peptide acceptor region which is bonded to the PEG region, to (b) a 3′ end of a nucleic acid comprising a 5′ untranslated region comprising a transcription promoter and a translation enhancer; an ORF region which comprises a polyA sequence and which is bonded to the 3′-terminal side of the 5′ untranslated region; and a 3′-terminal region that is bonded to the 3′-terminal side of the ORF region.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.