Method for purifying protein
US8283443B2 · kind B2 · utility
1Cited by
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9Claims
0Family size
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Key dates
| Filing date | Jul 9, 2009 |
| Grant date | Oct 9, 2012 |
| Priority date | — |
| Expiry date | Oct 29, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for purifying a protein, includes the step of: contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride. With this arrangement, it is possible to easily produce large quantity of proteins which are high in purity without sacrificing activity of the proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.