Patent · US Active

Cloning, expression and purification of recombinant porcine intrinsic factor for use in diagnostic assay

US8288124B2 · kind B2 · utility

0Cited by

16References

8Claims

0Family size

Assignee

Abbott Laboratories · US

Inventors

Ramani S. Wonderling · Waukegan, US
John F. Uher · Kenosha, US
Thomas P. Leary · Kenosha, US
You Pan · Hubei, CN

Key dates

Filing date	Nov 20, 2008
Grant date	Oct 16, 2012
Priority date	—
Expiry date	Nov 20, 2028

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/82
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The present invention relates to a new method of producing functionally active recombinant porcine Intrinsic Factor as well as the protein (i.e., porcine Intrinsic Factor) produced thereby. In particular, the vector comprising the DNA encoding the protein is introduced into a Chinese Hamster Ovary (CHO) host cell under conditions sufficient for optimal expression of functional protein. The expressed protein may then be subjected to three methods ultimately resulting in a protein having at least 97% purity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.