Methods for making proteins containing free cysteine residues
US8288126B2 · kind B2 · utility
6Cited by
85References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 18, 2011 |
| Grant date | Oct 16, 2012 |
| Priority date | — |
| Expiry date | Apr 18, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/005
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.