Biomolecular attachment sites on microelectronic arrays and methods thereof

US8288155B2 · kind B2 · utility

Assignee

• Gamida for Life B.V. · NL

Inventors

• John Havens · San Diego, US
• Thomas J. Onofrey · San Marcos, US
• Charles Greef · Louisville, US
• Gregory J. Kevorkian · Temecula, US
• Jain Krotz · San Diego, US
• Kristie L. Lykstad · San Diego, US
• Daniel E. Raymond · San Diego, US
• Howard Reese · Poway, US
• Regina Rooney · Village of La Jolla, US
• John Scott · Durham, GB

Key dates

• Filing date: Jul 13, 2007
• Grant date: Oct 16, 2012
• Expiry date: Mar 16, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/06
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods of addressing a biomolecule to a selectively addressable electrode are described. A permeation layer overlying a plurality of selectively addressable electrodes is provided. The permeation layer includes a reactive group that is adapted to bond to a biomolecule and that requires activation through a chemical transformation before bonding to the biomolecule. At least one selectively addressable electrode is biased such that a pH change occurs in an overlying solution of the at least one selectively addressable electrode. The reactive group in a portion of the permeation layer above the at least one selectively addressable electrode is then chemically transformed to an activated reactive group as a result of the pH change. A biomolecule is then bound to the permeation layer overlying the at least one selectively addressable electrode through the activated reactive group.