Method for high spatial resolution stochastic examination of a sample structure labeled with a substance

US8293490B2 · kind B2 · utility

Assignee

• LEICA MICROSYSTEMS CMS GMBH · DE

Inventors

• Volker Seyfried · Schallstadt, DE
• Jochen Sieber · Mannheim, DE

Key dates

• Filing date: Dec 7, 2009
• Grant date: Oct 23, 2012
• Expiry date: Jan 26, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/6458
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for high spatial resolution stochastic examination of a biological sample structure labeled with a labeling substance is described. The method comprises providing a biological sample structure; choosing such a labeling substance that has molecules present in a first state and in a second state, and the first and second states differ from one another in at least one photophysical property such that there is sufficient probability that one portion of the molecules of the substance will be in the first state and another portion of the molecules will be in the second state and within which labeling substance a change of the state of the molecules can occur spontaneously between the two states in both directions; and labeling the biological sample structure with the substance.