Method for inducing differentiation of pluripotent stem cells into cardiomyocytes
US8293529B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 27, 2007 |
| Grant date | Oct 23, 2012 |
| Priority date | — |
| Expiry date | Jun 13, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for inducing differentiation of cardiomyocytes efficiently and selectively from stem cells.A method for inducing differentiation of cardiomyocytes from pluripotent stem cells, which comprises: (i) culturing the pluripotent stem cells in a culture medium containing no substance that promotes activation of the canonical Wnt signaling pathway during the time period between initiation of differentiation induction and 24 hours before the period of elevated canonical Wnt gene expression; and then (ii) culturing the pluripotent stem cells in a culture medium containing a substance that promotes activation of the canonical Wnt signaling pathway during a time period of 24 to 96 hours, starting from 24 to 0 hours before the period of elevated canonical Wnt gene expression.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.