Selection method for cell internalizing nucleic acids

US8298764B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Andrew Ellington · Austin, US
• Matthew Levy · New Rochelle, US
• Amy Yan · Larchmont, US
• Ted Chitai Chu · Palo Alto, US

Key dates

• Filing date: Apr 9, 2008
• Grant date: Oct 30, 2012
• Expiry date: Oct 9, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1048
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention includes compositions and methods for contacting one or more cells with a random RNA-containing library, treating the contacted cells and with a denaturing agent or digestion with one or more nucleases, and extracting from the cells one or more internalized nucleic acids resistant to the nucleases or denaturants.