Reversible MHC multimer staining for functional purification of antigen-specific T cells

US8298782B2 · kind B2 · utility

Assignee

• IBA GMBH · JP

Inventors

• Dirk Busch · Munich, DE
• Hermann Wagner · Unterschleißheim, DE

Key dates

• Filing date: Jul 26, 2010
• Grant date: Oct 30, 2012
• Expiry date: Jul 26, 2030

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2239/38
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to a new method for reversible staining and functional isolation or characterization of cells, e.g. antigen-specific T cells. With this technique, the original functional status of cells can be substantially maintained after their identification and purification. Thus, this new method is of broad benefit for basic research and clinical applications.