Micro-organisms for the production of 1,2-propanediol obtained by a combination of evolution and rational design
US8298807B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 21, 2008 |
| Grant date | Oct 30, 2012 |
| Priority date | — |
| Expiry date | Jan 23, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B10/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of 1,2-propanediol from a carbon source. The said method comprises growing an initial strain under selection pressure in an appropriate growth medium, said initial bacterial strain comprising an attenuation of the expression of the tpiA gene and an attenuation the expression of at least one gene involved in the conversion of methylglyoxal to lactate, in order to promote evolution in said initial strain; then selecting and isolating the evolved strain having an increased 1,2 propanediol production rate; then reconstructing a functional tpiA gene in the evolved strain. The present invention also concerns the evolved strain such as obtained, that may be furthermore genetically modified in order to optimize the conversion of a carbon source into 1,2-propanediol without bv-products and with the best possible yield.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.