Method for identifying the elution time of an analyte
US8298829B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 25, 2011 |
| Grant date | Oct 30, 2012 |
| Priority date | — |
| Expiry date | Jan 25, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/8644
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.