Centrifugal microfluidic system for nucleic acid sample preparation, amplification, and detection

US8303911B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jonathan Siegrist · Walnut Creek, US
• Robert Gorkin · Exton, US
• Regis Peytavi · Cabestany, FR
• Marc J. Madou · San Diego, US
• Horacio Kido · Davis, US
• Mary Amasia · Irvine, US
• Emmanuel Roy · Paris, FR
• Teodor Veres · Montréal, CA

Key dates

• Filing date: Oct 14, 2010
• Grant date: Nov 6, 2012
• Expiry date: Oct 14, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N35/00069
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A microfluidic system for processing a sample includes a microfluidic CD in the form a rotatable disc, the disc containing a plurality of separate lysis chambers therein. A magnetic lysis blade and lysis beads are disposed in each of the lysis chambers and a plurality of stationary magnets are disposed adjacent to and separate from the microfluidic CD. The stationary magnets are configured to magnetically interact with each of the magnetic lysis blades upon rotation of the microfluidic CD. Each lysis chamber may have its own separate sample inlet port or, alternatively, the lysis chambers may be connected to one another with a single inlet port coupled to one of the lysis chambers. Downstream processing may include nucleic acid amplification using thermoelectric heating as well as detection using a nucleic acid microarray.