Selective terminal tagging of nucleic acids
US8304183B2 · kind B2 · utility
56Cited by
5References
12Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Nov 30, 2005 |
| Grant date | Nov 6, 2012 |
| Priority date | — |
| Expiry date | Oct 28, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for adding a terminal sequence tag to nucleic acid molecules for use in RNA or DNA amplification. The tag introduced may be used as a primer binding site for subsequent amplification of the DNA molecule and/or sequencing of the DNA molecule and therefore provides means for identification and cloning of the 5′-end or the complete sequence of mRNAs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.