Blood coagulation assays

US8304205B2 · kind B2 · utility

Assignee

• Siemens Healthcare Diagnostics Products GmbH · DE

Inventors

• Andreas Kappel · Höhenkirchen-Siegertsbrunn, DE
• Andrea Lichte · Marburg, DE
• Norbert Zander · Marburg, DE
• Stefan Teigelkamp · Niederwalgern, DE
• Carsten Schelp

Key dates

• Filing date: Sep 29, 2009
• Grant date: Nov 6, 2012
• Expiry date: Jun 7, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/974
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to methods for determining the activity of a proteolytic coagulation factor of the blood coagulation cascade in a body fluid such as whole blood or plasma. A combination is provided in a reaction mixture. The combination comprises the sample and an activation agent for activating a proteolytic coagulation factor of the blood coagulation cascade or for activating the blood coagulation cascade. The effect of the activating on a reagent system comprising a cleavable moiety is evaluated. The cleavable moiety is or becomes bound to a chemiluminescent agent or a sensitizer agent or both. The chemiluminescent agent and the sensitizer agent are related in that, when in close proximity, energization of the sensitizer agent results in energization of the chemiluminescent agent. The effect of the activating is related to the activity of a proteolytic coagulation factor of the blood coagulation cascade wherein the effect is the extent of cleavage of the cleavable moiety.