Method for the secretory production of heterologous protein in Escherichia coli
US8304210B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 6, 2009 |
| Grant date | Nov 6, 2012 |
| Priority date | — |
| Expiry date | Jul 6, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a signal sequence peptide for the improvement of extracellular secretion efficiency of a heterologous protein in E. coli. More particularly, the present invention relates to a gene construct for the improvement of extracellular secretion efficiency of the said heterologous protein in E. coli, which comprises a polynucleotide encoding a recombinant protein composed of the heterologous protein linked to C-terminal of the signal sequence peptide represented by SEQ. ID. NO. 3. The present invention contributes to the improvement of extracellular secretion efficiency of a recombinant protein, so that it can be effectively applied to the production of a recombinant protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.