Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US8304233B2 · kind B2 · utility
Assignees
Inventor
Key dates
| Filing date | Aug 4, 2005 |
| Grant date | Nov 6, 2012 |
| Priority date | — |
| Expiry date | May 14, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8509
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided system includes a site-specific integrating expression cassette (INTEC) vector, consisting of (a) a polynucleotide of interest operably linked to a promoter, (b) a single recombination site, and (c) a hybrid recombination site. In using the subject systems for site-specific integration, the INTEC vector and integrase are introduced into the target cell and the cell is maintained under conditions sufficient to provide for site-specific integration of the nucleic acid into the target cell genome via a recombination event mediated by the site-specific recombinase. Also provided are kits that include the subject systems. The subjects systems, methods and kits find use in a variety of different applications, several representative ones of which are described in detail as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.