High yield dialysis-free process for producing organosoluble regenerated silk fibroin
US8309689B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 20, 2010 |
| Grant date | Nov 13, 2012 |
| Priority date | — |
| Expiry date | Jan 7, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/43586
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a simple and high yield process for producing a regenerated silk fibroin which does not need dialysis. Particularly, a process of the invention is characterized in that the silk fibroin is precipitated by applying a shear stress and/or changing the solvent power of the fibroin solution. The process of the invention simplifies the process of producing silk fibroin and greatly shortens the process time to 1 to 2 hours, whereas the conventional dialysis process is complex and needs around 2 to 3 days. In addition to reducing the time needed, the process of the invention can increase productivity of silk fibroin by at least 8%.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.