Method of preparing a separation matrix

US8309709B2 · kind B2 · utility

Assignee

• GE Healthcare Bio-Science AB · US

Inventors

• Hans Berg · Uppsala, SE
• Maria Brannholm · Uppsala, SE
• David Buckley · Rochester, US
• Philippe Busson · Nantes, FR
• Anders Hagvall · Uppsala, SE
• Eva Holmgren · Uppsala, SE
• Henrik Ihre · Uppsala, SE
• Anders Larsson · Uppsala, SE
• Dag Lindstrom

Key dates

• Filing date: Jun 28, 2006
• Grant date: Nov 13, 2012
• Expiry date: Sep 20, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08J2301/00
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of preparing an insoluble separation matrix, which method comprises salt-treatment of a polysaccharide gel followed by cross-linking of the polysaccharide polymers. In one embodiment, the method comprises providing an aqueous solution of a gelatable native polysaccharide; lowering the temperature of the polysaccharide solution to a value below its gelling point; salt-treatment by adding at least one salt to the resulting polysaccharide; and cross-linking the salt-treated polysaccharide. The polysaccharide may be prepared e.g. into particles, membranes or monoliths.