High-throughput assay for evaluating dipeptidyl peptidase I activity
US8313920B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Oct 21, 2009 |
| Grant date | Nov 20, 2012 |
| Priority date | — |
| Expiry date | Sep 10, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of screening a compound which modulates dipeptidyl peptidase I (DPPI) activities comprises the steps of adding a peptide substrate of DPPI to a reaction mixture which comprises DPPI and a compound, wherein the peptide substrate of DPPI has at least 3 amino acids and binds to a binding site of DPPI in addition to the S1-S2 site; and measuring the molecular weight of the substrate, wherein a change in the molecular weight of the substrate is indicative of the presence of DPPI activity.
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