System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts
US8314222B2 · kind B2 · utility
0Cited by
7References
34Claims
0Family size
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Key dates
| Filing date | Oct 6, 2008 |
| Grant date | Nov 20, 2012 |
| Priority date | — |
| Expiry date | Oct 21, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2800/206
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.