Aptamer-based assays
US8318438B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 27, 2008 |
| Grant date | Nov 27, 2012 |
| Priority date | — |
| Expiry date | Aug 4, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/195
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.