Patent · US Active

Nucleic acid hybridization methods

US8318919B2 · kind B2 · utility

3Cited by
16References
4Claims
0Family size

Assignee

Inventor

Key dates

Filing dateMay 11, 2009
Grant dateNov 27, 2012
Priority date—
Expiry dateNov 18, 2030

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6837
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed herein are methods of destabilizing double-stranded nucleic acid hybridization using an enzyme comprising DNA N-glycosylase activity. Also disclosed herein is the detection of a double-stranded target DNA wherein the hybridization of duplex strands has been at least partially disrupted thereby permitting invasion of a probe strand. Also disclosed herein are methods of using an enzyme comprising DNA N-glycosylase activity to generate single-stranded circular nucleic acids.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.