Quantification of nucleic acid molecules using multiplex PCR

US8321140B2 · kind B2 · utility

Assignee

• PRIMERADX, INC. · US

Inventors

• Kyle Hart · Puyallup, US
• Vladimir Dancik · North Andover, US
• Michael Bristol · Foxborough, US
• Patrick D. Wells · North Oaks, US
• Elizabeth Garcia · Springfield, US
• Vladimir I. Slepnev · Newton, US
• John T. Unger · Medfield, US

Key dates

• Filing date: Nov 6, 2008
• Grant date: Nov 27, 2012
• Expiry date: Sep 12, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6851
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Described are novel quantification methods and systems that permit, within the context of multiplex PCR, the quantification of all targets within a single reaction tube. The methods employ quantitation algorithms applied to the amplification profiles of internal calibration controls or standards utilizing a plurality of nucleic acid templates that are amplified within the same reaction tube as the nucleic acid target(s) interrogated.