IC-processed polymer nano-liquid chromatoraphy system on-a-chip and method of making it
US8323488B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 28, 2011 |
| Grant date | Dec 4, 2012 |
| Priority date | — |
| Expiry date | Feb 28, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/645
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, flits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals. A self-aligned channel-strengthening technique increases pressure rating of the microfluidic system, allowing it to withstand the high pressure normally used in high performance liquid chromatography (HPLC). On-chip sample injection, separation, and detection of mixture of anions in water is successfully demonstrated using ion-exchange nano-LC.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.