Methods for detecting nucleic acid sequence variations
US8323929B2 · kind B2 · utility
4Cited by
59References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 7, 2009 |
| Grant date | Dec 4, 2012 |
| Priority date | — |
| Expiry date | Oct 18, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.