Patent · US Active

Histone demethylation mediated by the nuclear amine oxidase homolog LSD1

US8323941B2 · kind B2 · utility

1Cited by

0References

15Claims

0Family size

Assignee

President and Fellows of Harvard College · US

Inventors

Yang Shi · Lo Wu, CN
Yujiang Geno Shi · Sharon, US

Key dates

Filing date	Apr 5, 2010
Grant date	Dec 4, 2012
Priority date	—
Expiry date	Nov 10, 2030

Classification

Technology area (CPC G)Physics
CPC primaryG01N2500/02
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

LSD1, a homolog of nuclear amine oxidases, functions as a histone demethylase and transcriptional co-repressor. LSD1 specifically demethylates histone H3 lysine 4, which is linked to active transcription. Lysine demethylation occurs via an oxidation reaction that generates formaldehyde. Importantly, RNAi inhibition of LSD1 causes an increase in H3 lysine 4 methylation and concomitant de-repression of target genes, suggesting that LSD1 represses transcription via histone demethylation. The results thus identify a histone demethylase conserved from S. pombe to human and reveal dynamic regulation of histone methylation by both histone methylases and demethylases.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.