Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm
US8326547B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 30, 2010 |
| Grant date | Dec 4, 2012 |
| Priority date | — |
| Expiry date | Jan 22, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B25/00
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5′-UTR, ORF and 3′-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.