Glucose dehydrogenase
US8329439B2 · kind B2 · utility
Assignees
Inventor
Key dates
| Filing date | Jun 23, 2008 |
| Grant date | Dec 11, 2012 |
| Priority date | — |
| Expiry date | Sep 2, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y101/05002
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.