Method for the cultivation of primary cells and for the amplification of viruses under serum free conditions

US8329466B2 · kind B2 · utility

Assignee

• Bavarian Nordic A/S · DK

Inventors

• Ingmar Rathe · Olching, DE
• Eva Felder · Munich, DE
• Karl Heller · Unterföhring, DE

Key dates

• Filing date: May 12, 2011
• Grant date: Dec 11, 2012
• Expiry date: May 12, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/24151
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for the cultivation of primary cells. The primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors.The method for the cultivation of primary cells may be one step in a method for the amplification of viruses, such as poxviruses. According to this latter method the primary cells are cultivated in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors. The cells are then infected with the virus and the infected cells are cultivated in serum free medium until progeny virus is produced.