Methods for protein refolding
US8329878B2 · kind B2 · utility
4Cited by
26References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 17, 2009 |
| Grant date | Dec 11, 2012 |
| Priority date | — |
| Expiry date | Jun 14, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/2462
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses improved methods of disaggregating protein aggregates, and refolding denatured proteins, using high pressure. In particular, the present invention provides for the use of agitation, high temperature, “stepped” depressurization, dialysis and dilution under pressure to increase the speed and extent of aggregate dissolution and protein refolding.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.