Patent · US Active

Methods and means for obtaining modified phenotypes

US8334374B2 · kind B2 · utility

7Cited by

102References

19Claims

0Family size

Assignee

COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION · AU

Inventors

Peter Michael Waterhouse · Canberra, AU
Ming-Bo Wang · Canberra, AU

Key dates

Filing date	May 17, 2012
Grant date	Dec 18, 2012
Priority date	—
Expiry date	May 17, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y302/01031
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eukaryotic cells, particularly in plant cells, by providing aberrant, preferably unpolyadenylated, target-specific RNA to the nucleus of the host cell. Preferably, the unpolyadenylated target-specific RNA is provided by transcription of a chimeric gene comprising a promoter, a DNA region encoding the target-specific RNA, a self-splicing ribozyme and a DNA region involved in 3′ end formation and polyadenylation.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.