Method for the quantitative determination of the concentration of fluorophores of a substance in a sample and apparatus for carrying out the same
US8334522B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 12, 2009 |
| Grant date | Dec 18, 2012 |
| Priority date | — |
| Expiry date | Mar 17, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6428
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns methods and apparatuses for quantitatively determining the concentration of fluorophores of a substance in a sample. A constant portion of the reference light of a reference light wave length (λr) emitted by a reference light source is coupled in by an optical element in the direction of a receiving element. A first value corresponding to the portion of the reference light coupled in which is incident on the receiving element is detected. The sample is irradiated with the excitation light of an excitation wave length (λex) emitted by an excitation light source. A second value corresponding to the portion of the fluorescent light of an emission wave length (λem) emitted by the sample which is incident on the receiving element. The ratio of the second value to the first value is determined. The number of fluorophores in the substance is determined based on the ratio.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.