Manipulation of magnetic microparticles in a high pressure liquid system and extraction process
US8337704B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 4, 2008 |
| Grant date | Dec 25, 2012 |
| Priority date | — |
| Expiry date | Feb 10, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2446/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention concerns a device and a method for the manipulation of a liquid sample material in which magnetic microparticles are suspended whereby the microparticles have a functionalized surface and an analyte is bound to the surface. The sample material is introduced into a device with a liquid system through an injection device (50) and in a first mobile phase the sample material is carried to an extractor (90). In a first section (97a) of the extractor (90) the microparticles are immobilized by means of a magnetic field of a controllable device (96) and separated from the remaining sample material. By switching over of a switching unit (110) a second mobile phase (75) is carried to the extractor (90) and the second mobile phase (75) detaches the adsorbed analyte from the surface of the microparticles. In a second section (97b) of the extractor (90) the microparticles are immobilized and the second mobile phase (75) with the dissolved analyte(s) can be analyzed by way of chromatographic separation (130) and subsequent detection (140).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.