Engineered human broncho-epithelial tissue-like assemblies

US8338114B1 · kind B1 · utility

Assignee

• The United States of America as represented by the Administrator of the National Aeronautics and Space Administration · US

Inventor

• Thomas J. Goodwin · Friendswood, US

Key dates

• Filing date: Apr 19, 2007
• Grant date: Dec 25, 2012
• Expiry date: May 4, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2531/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Three-dimensional human broncho-epithelial tissue-like assemblies (TLAs) are produced in a rotating wall vessel (RWV) with microcarriers by coculturing mesenchymal bronchial-tracheal cells (BTC) and bronchial epithelium cells (BEC). These TLAs display structural characteristics and express markers of in vivo respiratory epithelia. TLAs are useful for screening compounds active in lung tissues such as antiviral compounds, cystic fibrosis treatments, allergens, and cytotoxic compounds.