Obligately anaerobic mutant lactic acid bacterium and preparation method therefor, and expression vector functioning in obligately anaerobic lactic acid bacterium
US8338162B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 17, 2009 |
| Grant date | Dec 25, 2012 |
| Priority date | — |
| Expiry date | Mar 31, 2031 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P43/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides an obligately anaerobic lactic acid bacterium having no risk of causing side effects in normal tissue and promising as a safe therapeutic agent and a gene transporter for a disease that is in an anaerobic environment such as a solid tumor, a preparation method therefor, and an expression vector useful in the preparation method. The obligately anaerobic lactic acid bacterium of the present invention has been artificially mutated from being facultatively anaerobic to being obligately anaerobic and, furthermore, is capable of being transformed by an expression vector having introduced thereinto a gene for expressing an active protein useful for the treatment of a disease that is in an anaerobic environment. Furthermore, the expression vector of the present invention functions in an obligately anaerobic lactic acid bacterium and contains a Lactobacillus-derived plasmid replication protein gene (Rep), a secretion signal sequence (PslpA-SSartP) comprising a Lactobacillus-derived s-layer gene promoter and a Lactobacillus-derived PrtP protein secretion signal, and one or more selection marker genes. The expression vector of the present invention enables produ…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.