Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture

US8338166B2 · kind B2 · utility

Assignee

• Lawrence Livermore National Security, LLC · US

Inventors

• Neil Reginald Beer · Pleasanton, US
• Benjamin Hindson · Pleasanton, US
• Billy W. Colson, Jr. · San Ramon, US
• Joseph P. Fitch · Livermore, US

Key dates

• Filing date: Jan 4, 2007
• Grant date: Dec 25, 2012
• Expiry date: Sep 29, 2031

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0487
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A system for identifying all of the known and unknown pathogenic or non-pathogenic organisms in a sample. A droplet generator creates droplets from the sample. The droplets constitute sub-nanoliter volume reactors containing the organism sized particles. A lysis device performs lysis of the organisms to release the nucleic acids. An amplifier amplifies the nucleic acids. A fractionater releases the nucleic acids from the droplets. A parallel analyzer identifies all of the known and unknown pathogenic or non-pathogenic organisms in the sample.