Cell/ligand marking system, wherein the marker is of Eph type, cell material comprising said system, method for preparing same and proangiogenetic use

US8338169B2 · kind B2 · utility

Assignees

• Institut des Vaisseaux et du Sang · FR
• Universite Paris 7—Denis Diderot 2 · FR

Inventors

• Philippe Foubert · Boulogne-Billancourt, FR
• Sophie Le Ricousse-Roussanne · Champigny-sur-Marne, FR
• Jean-Sebastien Silvestre · Paris, FR

Key dates

• Filing date: Jul 27, 2006
• Grant date: Dec 25, 2012
• Expiry date: Jul 7, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A cell/ligand specific marking system, the system being characterized in that it comprises: (a) an endothelial precursor cell (EPC) including a cell marker selected from among the group consisting of the Eph (in particular EphB4 or EphB1), and (b) a protein material of structure L-K, which consists of a ligand (L) specific of the marker and is associated or fused with a binding protein (K), the system being capable of providing a proangiogenetic cell material of structure EPC-Eph-L-K. The invention also concerns the cell material as product capable of stimulating angiogenesis, its preparation method and its therapeutic use, in particular with respect to vascular insufficiencies.