Metabolically engineered cells for the production of pinosylvin

US8343739B2 · kind B2 · utility

Assignee

• Fluxome Sciences A/S

Inventors

• Michael Katz · Malmö, SE
• Jochen Förster · Copenhagen, DK
• Helga David · Middelfart, DK
• Hans-Peter Schmidt · Echterdingen, DE
• Malin Sendelius · Lund, SE
• Sara Peterson Bjørn · Avenue Madeleine, DK
• Thomas Durhuus · Middelfart, DK

Key dates

• Filing date: Jul 19, 2007
• Grant date: Jan 1, 2013
• Expiry date: Dec 19, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/93
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.