Annealing curve analysis in PCR
US8343754B2 · kind B2 · utility
39Cited by
10References
14Claims
0Family size
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Key dates
| Filing date | May 7, 2012 |
| Grant date | Jan 1, 2013 |
| Priority date | — |
| Expiry date | May 7, 2032 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.