Multi-color fluorescence enhancement from a photonic crystal surface
US8344333B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jun 28, 2011 |
| Grant date | Jan 1, 2013 |
| Priority date | — |
| Expiry date | Jul 11, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/7743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, which can be fabricated uniformly from plastic materials over a ˜3×5 in2 surface area by nanoreplica molding, features a 1-D periodic grating structure which utilizes two distinct resonant modes to enhance electric field stimulation of a first dye excited by a first laser (e.g., λ=632.8 nm laser exciting cyanine-5) and a second dye excited by a second laser (e.g., λ=532 nm laser exciting cyanine-3). The first and second lasers could be replaced by a single variable wavelength (tunable) laser. Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle θ. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in a given wavelength range (e.g., the range 532 nm<λ<660 nm) by selection of an appropriate incident angle. The device can be used for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.