Laser dosimetry for the optoperforation of single cells
US8349598B2 · kind B2 · utility
1Cited by
0References
19Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 9, 2008 |
| Grant date | Jan 8, 2013 |
| Priority date | — |
| Expiry date | Aug 6, 2029 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61B2018/00636
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
A method of optoperforation of the membrane of a cell by application of laser pulses characterized by focusing the pulsed laser beam onto the cell membrane to be perforated, applying a series of laser pulses of predetermined pulse energy, measuring the oscillation time of the bubbles formed in the laser focus from the change in laser intensity of a test laser beam transmitted through the laser focus and caused by the bubbles in the laser focus, and increasing the pulse energy to a level at which the oscillation time of the bubbles attains a predetermined value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.