Method for the optimization of chromatographic purification processes for biological molecules

US8349615B2 · kind B2 · utility

Assignee

• BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG · DE

Inventors

• Christian Eckermann · Biberach, DE
• Sybille Ebert · Biberach, DE
• Stefanie Rubenwolf · Nattenhausen, DE
• Dorothee Ambrosius · Munich, DE

Key dates

• Filing date: Jun 13, 2007
• Grant date: Jan 8, 2013
• Expiry date: Jan 7, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/009
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method of finding suitable parameters for the chromatographic purification of biomolecules. The method consists of equilibration, charging, washing and eluting steps, this sequence of steps being carried out by the partial batch method. The parameters determined in small, preferably numerous parallel test batches provide conclusions as to the chromatography conditions under which a given biomolecule can be purified optimally by column chromatography, optionally even on a larger scale.