Methods for calibrating a fluorometer
US8352207B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 31, 2010 |
| Grant date | Jan 8, 2013 |
| Priority date | — |
| Expiry date | Jul 12, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0221
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Some embodiments provide methods for calibrating a fluorometer in order to account for one or more optical properties of a water sample affecting fluorescence measurements. In some cases one or more calibration solutions are prepared with sample water from a specific field site. Fluorescence measurements are taken from a water sample and one or more of the calibration solutions, and calibration parameters are determined based on the measurements. In some cases a calibration solution is prepared by spiking sample water to include a higher concentration of a fluorescent tracer and measurements are taken to characterize a calibration slope coefficient. In some cases a calibration solution is prepared by adding an acid and measurements are taken to characterize a background fluorescence in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.