Glucose dehydrogenase/cytochrome fusion protein
US8354112B2 · kind B2 · utility
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Key dates
| Filing date | Sep 28, 2004 |
| Grant date | Jan 15, 2013 |
| Priority date | — |
| Expiry date | Mar 11, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A fusion protein of pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) and a cytochrome is disclosed. PQQGDH is, for example, a water-soluble PQQGDH derived from Acinetobacter calcoaceticus. The cytochrome is, for example, an electron transfer domain of quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni. The fusion protein of the present invention shows intramolecular electron transfer from PQQ, a redox center, to the cytochrome, which allow construction of a direct electron transfer-type glucose sensor which requires no electron mediators.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.