T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
US8354232B2 · kind B2 · utility
2Cited by
66References
11Claims
0Family size
Assignee
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Key dates
| Filing date | Jul 20, 2010 |
| Grant date | Jan 15, 2013 |
| Priority date | — |
| Expiry date | Feb 22, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.