Protein glycosylation modification in methylotrophic yeast
US8354268B2 · kind B2 · utility
12Cited by
4References
21Claims
0Family size
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Key dates
| Filing date | Sep 25, 2003 |
| Grant date | Jan 15, 2013 |
| Priority date | — |
| Expiry date | May 27, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/52
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides genetically engineered strains of Pichiacapable of producing proteins with reduced glycosylation. In particular, the genetically engineered strains of the present invention are capable of expressing either or both of an α-1,2-mannosidase and glucosidase II. The genetically engineered strains of the present invention can be further modified such that the OCH1 gene is disrupted. Methods of producing glycoproteins with reduced glycosylation using such genetically engineered stains of Pichia are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.