Integrated instrument performing synthesis and amplification, and a system and method thereof

US8357490B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Thomas Froehlich · Ottikon, CH
• Martin Gutekunst · Eberfing, DE
• Dieter Heindl · Munich, DE
• Angelika Roesler · Sindelsdorf, DE
• Rudolf Seibl · Penzberg, DE

Key dates

• Filing date: Jul 22, 2010
• Grant date: Jan 22, 2013
• Expiry date: Jul 22, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/00356
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An integrated instrument for oligonucleotide synthesis and PCR, and a system and method thereof are disclosed. The integrated instrument is basically composed of two independent modules. The first module is a unit for chemical de novo synthesis of oligonucleotides such as oligonucleotide primers and/or oligonucleotide hybridization probes. The second module is a unit for performing an analytical polymerase chain reaction amplification in real time, i.e. a qPCR. The two modules are operatively linked to each other in such a way that a user can load a nucleic sample to be analyzed into the integrated instrument and perform a PCR reaction by programming the instrument without a previous external synthesis of oligonucleotide amplification primers.